Purification, characterization and utilization of polysaccharide of Araucaria heterophylla gum for the synthesis of curcumin loaded nanocarrier
| Autor: | R. Preethi, J. Lavanya Agnes Angalene, S. M. Roshini, Raji P, Ponnaiah Paulraj, S. Suresh Kumar, Antony V. Samrot, S. M. Stefi, A. Madan Kumar |
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| Rok vydání: | 2019 |
| Předmět: |
Arabinose
Curcumin Araucaria Rhamnose Glucosinolates Antineoplastic Agents 02 engineering and technology Polysaccharide Biochemistry Antioxidants 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Polysaccharides Structural Biology Neoplasms Spectroscopy Fourier Transform Infrared Humans Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography Threose Chemistry Galactose General Medicine 021001 nanoscience & nanotechnology Solvent Glucose MCF-7 Cells Allose Nanocarriers Tetroses 0210 nano-technology Antibacterial activity |
| Zdroj: | International Journal of Biological Macromolecules. 140:393-400 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.08.121 |
| Popis: | In this study, gum of Araucaria heterophylla was collected. The collected gum was subjected for extraction of polysaccharide using solvent extraction system. Thus, extracted polysaccharide was further purified using solvent method and was characterized using UV-Vis spectroscopy, Phenol sulfuric acid assay, FTIR, TGA, TLC and GC-MS. The gum derived polysaccharide was found to have the following sugars Rhamnose, Allose, Glucosinolate, Threose, Idosan, Galactose and Arabinose. The extracted polysaccharide was tested for various in-vitro bioactive studies such as antibacterial activity, antioxidant activity and anticancer activity. The polysaccharide was found to have antioxidant and anticancer activity. Further, the polysaccharide was subjected for carboxymethylation to favor the nanocarrier synthesis, where it was chelated using Sodium Tri Meta Phosphate (STMP) to form nanocarriers. The nanocarriers so formed were loaded with curcumin and were characterized using FTIR, SEM, EDX and AFM. Both the loaded and unloaded nanocarriers were studied for its in-vitro cytotoxic effect against the MCF7 human breast cancer cell lines. The nanocarriers were found to deliver the drug efficiently against the cancer cell line used in this study. |
| Databáze: | OpenAIRE |
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