Factors affecting the efficiency of embryo transfer in the domestic ferret (Mustela putorius furo)
| Autor: | Ziyi Li, Juan Chen, Gregory H. Leno, John F. Engelhardt, Xingshen Sun |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Conservation of Natural Resources
Nuclear Transfer Techniques medicine.medical_specialty Cloning Organism Uterus Embryonic Development Reproductive technology Biology Article Andrology Food Animals Pregnancy biology.animal medicine Animals Mink Small Animals Gynecology Fetus Equine Ferrets Embryo Embryo Transfer medicine.disease Embryo transfer Disease Models Animal Parity medicine.anatomical_structure embryonic structures Tissue and Organ Harvesting Somatic cell nuclear transfer Female Animal Science and Zoology |
| Zdroj: | Theriogenology. 66:183-190 |
| ISSN: | 0093-691X |
| Popis: | Embryo transfer (ET) to recipient females is a foundational strategy for a number of assisted reproductive technologies, including cloning by somatic cell nuclear transfer. In an attempt to develop efficient ET in domestic ferrets, factors affecting development of transferred embryo were investigated. Unilateral and bilateral transfer of zygotes or blastocysts in the oviduct or uterus was evaluated in recipient nulliparous or primiparous females. Developing fetuses were collected from recipient animals 21 days post-copulation and examined. The percentage of fetal formation was different (P < 0.05) for unilateral and bilateral transfer of zygotes (71%) in nulliparous females with bilateral transfer (56%) in primiparous recipients. The percentage (90%) of fetal formation in nulliparous recipients following unilateral transfer of blastocysts was higher (P < 0.05) than that observed in primiparous recipients with bilateral ET (73%). Notably, the percentage of fetal formation was higher (P < 0.05) when blastocyts were transferred as compared to zygotes (90% versus 71%). Transuterine migration of embryos occurred following all unilateral transfers and also in approximately 50% of bilateral transfers with different number of embryos in each uterine horn. These data will help to facilitate the development of assisted reproductive strategies in the ferret and could lead to the use of this species for modeling human disease and for conservation of the endangered Mustelidae species such as black-footed ferret and European mink. |
| Databáze: | OpenAIRE |
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