Transformation of adult ventricular myocytes with the temperature sensitive A58 (tsA58) mutant of the SV40 large T antigen
| Autor: | Jill Rulfs, Michael Allan Buckholt, Caroline Miller, Stephen R. Jaspers, Thomas B. Miller |
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| Rok vydání: | 1994 |
| Předmět: |
Male
SV40 large T antigen Cell division Antigens Polyomavirus Transforming Heart Ventricles Recombinant Fusion Proteins Cellular differentiation Transgene Genetic Vectors Clinical Biochemistry Simian virus 40 Myosins Biology Transfection Rats Sprague-Dawley Myosin Animals Molecular Biology Cells Cultured Contact Inhibition Age Factors Temperature Contact inhibition Cell Differentiation Cell Biology General Medicine Cell Transformation Viral In vitro Rats Cell biology Immunology Cell Division |
| Zdroj: | Molecular and Cellular Biochemistry. 136:29-34 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | Freshly isolated ventricular myocytes have been used extensively as an adult cardiac model system. Due to their inability to undergo cytokinesis in vitro and their dedifferentiated properties in long-term culture, they can not be used for extended studies. Recent reports tell of the establishment of fetal and neonatal cardiac cell lines and the development of adult cardiomyocytes from transgenic animals. A recent report by Kirshenbaum [1], is the first to demonstrate insertion of genes in to adult ventricular myocytes using viral infection. This paper discusses the infection of primary adult differentiated cardiomyocytes with the SV40 large T antigen and subsequent proliferation under temperature sensitive control. Upon further characterization, the cells could be used as a model to study muscle differentiation and repair as well as adult cardiac cell physiology. |
| Databáze: | OpenAIRE |
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