Genome analysis of Cronobacter phage vB_CsaP_Ss1 reveals an endolysin with potential for biocontrol of Gram-negative bacterial pathogens
Autor: | Olivia McAuliffe, R. Paul Ross, Caitriona M. Guinane, Jim O'Mahony, Christopher D Johnston, Horst Neve, Lorraine Endersen, Aidan Coffey |
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Rok vydání: | 2015 |
Předmět: |
Sequence analysis
Molecular Sequence Data Lysin Genome Viral Peptidoglycan Genome Microbiology Bacteriophage Open Reading Frames chemistry.chemical_compound Cell Wall Virology Endopeptidases Gram-Negative Bacteria Bacteriophages ORFS Cronobacter Pest Control Biological Base Composition biology Hydrolysis Sequence Analysis DNA biology.organism_classification Open reading frame chemistry DNA Viral |
Zdroj: | Journal of General Virology. 96:463-477 |
ISSN: | 1465-2099 0022-1317 |
DOI: | 10.1099/vir.0.068494-0 |
Popis: | Bacteriophages and their derivatives are continuously gaining impetus as viable alternative therapeutic agents to control harmful multidrug-resistant bacterial pathogens, particularly in the food industry. The reduced efficacy of conventional antibiotics has resulted in a quest to find novel alternatives in the war against infectious disease. This study describes the full-genome sequence of Cronobacter phage vB_CsaP_Ss1, with subsequent cloning and expression of its endolysin, capable of hydrolysing Gram-negative peptidoglycan. Cronobacter phage vB_CsaP_Ss1 is composed of 42 205 bp of dsDNA with a G+C content of 46.1 mol%. A total of 57 ORFs were identified of which 18 could be assigned a putative function based on similarity to characterized proteins. The genome of Cronobacter phage vB_CsaP_Ss1 showed little similarity to any other bacteriophage genomes available in the database and thus was considered unique. In addition, functional analysis of the predicted endolysin (LysSs1) was also investigated. Zymographic experiments demonstrated the hydrolytic activity of LysSs1 against Gram-negative peptidoglycan, and this endolysin thus represents a novel candidate with potential for use against Gram-negative pathogens. |
Databáze: | OpenAIRE |
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