Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
| Autor: | Mahlet D Mersha, Karla R Sanchez, Murali K. Temburni, Harbinder S Dhillon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Bisphenol S General Chemical Engineering Physiology 030209 endocrinology & metabolism Development General Biochemistry Genetics and Molecular Biology Hazardous Substances 03 medical and health sciences chemistry.chemical_compound Issue 133 0302 clinical medicine Bisphenol A Endocrine disrupting compound Animals Humans Habituation Caenorhabditis elegans Behavior Non-associative learning General Immunology and Microbiology biology Behavior Animal General Neuroscience Reproduction Embryogenesis biology.organism_classification Fecundity 3. Good health 030104 developmental biology chemistry Lethality Endocrine Disrupting Compound Developmental biology hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | Bisphenols, such as bisphenol A (BPA) and bisphenol S (BPS) are polymerizing agents widely used in the production of plastics and numerous everyday-use products. Based on their chemical structure and estradiol-like biological properties, they have been classified as endocrine disrupting compounds (EDC). Long-term exposure to EDCs, even at low doses, has been linked to various health defects including cancer, behavioral disorders and infertility, with greater vulnerability indicated during early developmental periods. Cellular and molecular studies with the genetically tractable nematode model Caenorhabditis elegans have demonstrated that exposure to BPA causes apoptosis, embryonic lethality and disruption in the DNA repair mechanisms. We have previously reported that exposure of C. elegans embryos to low doses of different bisphenols decreases fecundity. In addition, we have shown that the effects of exposure during the very early stages of development persist into adulthood as assayed by quantifying habituation behavior, a form of non-associative learning. Here, we provide detailed protocols for embryonic exposure to low-dose EDCs as well as the associated fecundity and anterior touch habituation assays, along with representative results. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|