Seasonal photoperiodism regulates the expression of cuticular and signalling protein genes in the pea aphid
| Autor: | N. Prunier-Leterme, A. Benedetto, J.-C. Simon, G. Le Trionnaire, Stéphanie Jaubert, Denis Tagu, Beatriz Sabater-Muñoz, Joël Bonhomme, David Martínez-Torres |
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| Přispěvatelé: | Biologie des organismes et des populations appliquées à la protection des plantes (BIO3P), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universitat de València (UV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Cell signaling Photoperiod Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Complementary DNA Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Molecular Biology Gene Oligonucleotide Array Sequence Analysis 030304 developmental biology Genetics photoperiodism 0303 health sciences Aphid Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling food and beverages biology.organism_classification Sexual reproduction Acyrthosiphon pisum ADNc Gene Expression Regulation Aphids Insect Science Insect Proteins Female Seasons Head Moulting 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Insect Biochemistry and Molecular Biology Insect Biochemistry and Molecular Biology, Elsevier, 2007, 37 (10), pp.1094-1102. ⟨10.1016/j.ibmb.2007.06.008⟩ Insect Biochemistry and Molecular Biology, 2007, 37 (10), pp.1094-1102. ⟨10.1016/j.ibmb.2007.06.008⟩ |
| ISSN: | 0965-1748 |
| Popis: | International audience; Seasonal photoperiodism in aphids is responsible for the spectacular switch from asexual to sexual reproduction. However, little is known on the molecular and physiological mechanisms involved in reproductive mode shift through the action of day length. Earlier works showed that aphid head, but not eyes, directly perceives the photoperiodic signal through the cuticle. In order to identify genes regulating the photoperiodic response, a 3321 cDNA microarray developed for the pea aphid, Acyrthosiphon pisum was used to compare RNA populations extracted from heads of short- and long-day reared aphids. Microarray analyses revealed that 59 different transcripts were significantly regulated, among which a majority encoded cuticular proteins and several encoded proteins involved in cellular signalling or signal transduction. These results were confirmed by quantitative RT-PCR experiments on two cuticular and three signalling protein genes. Complementary experiments eliminated moulting and circadian rhythms as putative confounding effects. Quantitative RT-PCR performed at additional developmental stages demonstrated the regulation of expression of cuticular and signalling protein genes during the whole process of photoperiod shortening. This suggests that photoperiodic changes could affect cuticle structure and cell to cell communication in the head of aphids in relation with the switch of reproductive modes |
| Databáze: | OpenAIRE |
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