Characterization of a novel isoform of murine interferon regulatory factor 3
| Autor: | Yi Qin, Wei Liu, Jing Liu, Ran Hong, Jian-wei Zhai, Yuan Wang, Huafang Ouyang, Youhua Xie, Daming Gao, Yu-Ying Kong |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Transcriptional Activation
Gene isoform Transcription Genetic Newcastle Disease Biophysics Electrophoretic Mobility Shift Assay Biology Biochemistry Cell Line Mice Transcription (biology) Interferon medicine Animals Protein Isoforms Tissue Distribution Electrophoretic mobility shift assay RNA Messenger Promoter Regions Genetic Molecular Biology Gene Cell Nucleus Mice Inbred BALB C Reporter gene Interferon-beta Cell Biology Virology Cell biology Protein Transport Liver Cell culture Female Interferon Regulatory Factor-3 Interferon regulatory factors medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 377:384-388 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.09.147 |
| Popis: | Interferon (IFN) regulatory factors (IRFs) are a family of transcription mediators involved in the regulation of type I IFN (IFN-alpha/beta) transcription. Among the nine already identified IRFs, IRF-3 is a constitutively and ubiquitously expressed and plays a critical role in the transcriptional activation of type I IFN and IFN-stimulated genes including IFN-alpha1, IFN-beta and RANTES. In the present study, we report the identification of a novel alternatively spliced transcript of murine Irf-3 gene (mIrf-3) designated mIRF-3a. mIRF-3a is ubiquitously present in mouse tissues along with mIRF-3 and could be translocated into the nucleus in uninfected L929 cells. EMSA and reporter assay demonstrated that mIRF-3a binds to ISRE sequences in murine Ifn-beta promoter and represses Ifn-beta promoter activation induced by Newcastle disease virus infection. These results suggest that mIRF-3a may act as a modulator of mIRF-3 functions in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect