Type 2 diabetes differentially affects the substrate saturation kinetic attributes of erythrocyte hexokinase and phosphofructokinase
| Autor: | Sunita S Bhise, Mahabaleshwar V. Hegde, Janhavi R Rao, Surendra S. Katyare |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Erythrocytes Protein subunit Biophysics Type 2 diabetes Kinetic energy Biochemistry Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate Structural Biology Hexokinase Genetics medicine Humans Hypoglycemic Agents Enzyme kinetics Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030302 biochemistry & molecular biology Cell Biology Middle Aged medicine.disease Kinetics Enzyme Diabetes Mellitus Type 2 Phosphofructokinases chemistry Female Saturation (chemistry) Glycolysis Protein Binding Phosphofructokinase |
| Zdroj: | FEBS Letters. 594:240-250 |
| ISSN: | 1873-3468 0014-5793 |
| Popis: | The substrate kinetic parameters of hexokinase (HK) and phosphofructokinase (PFK)-the key irreversible enzymes of glycolysis-in erythrocytes from type 2 diabetic subjects were examined in comparison with control subjects. It was observed that the kinetic parameters such as Km , Vmax , Apparent Kcat , Kcat /Km , and substrate (ATP) inhibition kinetic and substrate binding characteristics are significantly altered in the diabetic group. The observed changes are suggestive of compositional changes in the subunit makeup of HK and PFK. The implication of these findings in relation to energy status of the diabetic erythrocyte and its interrelationship with loss of cell deformability are discussed here. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text