Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture
| Autor: | Tae Kwang Ha, Gyun Min Lee, Dong-Il Kim, Lise Marie Grav, Che Lin Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Glycosylation
medicine.drug_class Bioengineering CHO Cells Biology Monoclonal antibody Applied Microbiology and Biotechnology law.invention chemistry.chemical_compound Cricetulus High productivity law Cricetinae medicine Animals Chinese hamster ovary cell Therapeutic protein Antibodies Monoclonal Recombinant Proteins Cell biology Culture Media Titer chemistry Cell culture Batch Cell Culture Techniques Recombinant DNA Biotechnology |
| Zdroj: | Biotechnology advances. 54 |
| ISSN: | 1873-1899 |
| Popis: | Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the commercial production of therapeutic proteins. Fed-batch culture is widely used to produce therapeutic proteins, including monoclonal antibodies, because of its operational simplicity and high product titer. Despite technical advances in the development of culture media and cell cultures, it is still challenging to maintain high productivity in fed-batch cultures while also ensuring good product quality. In this review, factors that affect the quality attributes of therapeutic proteins in recombinant CHO (rCHO) cell culture, such as glycosylation, charge variation, aggregation, and degradation, are summarized and categorized into three groups: culture environments, chemical additives, and host cell proteins accumulated in culture supernatants. Understanding the factors that influence the therapeutic protein quality in rCHO cell culture will facilitate the development of large-scale, high-yield fed-batch culture processes for the production of high-quality therapeutic proteins. |
| Databáze: | OpenAIRE |
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