Nanoparticle-based 3D membrane for impedimetric biosensor applications
| Autor: | Lu Cao, Richard Luxton, Janice Kiely, Martina Piano |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
CRP detection
Materials science Scanning electron microscope Sonication Biophysics Analytical chemistry Impedance spectroscopy Nanoparticle Biosensing Techniques chemistry.chemical_compound Limit of Detection ZnO/CuO nitrocellulose membrane Electrochemistry Phase change Physical and Theoretical Chemistry Detection limit technology industry and agriculture Membranes Artificial General Medicine Dielectric spectroscopy Membrane chemistry Microscopy Electron Scanning Nanoparticles Biosensor Nitrocellulose |
| Zdroj: | Bioelectrochemistry. 136:107593 |
| ISSN: | 1567-5394 1878-562X |
| DOI: | 10.1016/j.bioelechem.2020.107593 |
| Popis: | This paper reports on a comparison between nano-ZnO/CuO and nano-ZnO nitrocellulose membrane biosensors, both of which were fabricated using a simple and inexpensive sonication technique. To produce the nano-ZnO/CuO membranes, the technique involved sonication of 1% (w/v) ZnO and 1% (w/v) CuO nano-crystal colloidal suspensions, with a volume ratio of 1:2. The membranes were analysed by scanning electron microscopy and energy-dispersive spectroscopy, which showed the gradated distribution of nanoparticles in the membrane. Impedance spectroscopy demonstrated that the sonication resulted in a greater than two-fold enhancement of the output signal. Changes in impedance phase values, at a frequency of 100 Hz, were used to establish dose dependent responses for C-reactive protein (CRP). Limits of detection of 27 pg/mL for the 1% (w/v) nano-ZnO and 16 pg/mL for the 1% (w/v) nano-ZnO/CuO nitrocellulose membrane biosensors were demonstrated. |
| Databáze: | OpenAIRE |
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