Engineered symbionts activate honey bee immunity and limit pathogens

Autor:	Jiri Perutka, Peng Geng, Andrew D. Ellington, Jeffrey E. Barrick, Bryan William Davies, J. Elijah Powell, Sean P. Leonard, Richard D. Horak, Nancy A. Moran, Luke C. Heckmann
Rok vydání:	2020
Předmět:	Genetics 0303 health sciences Multidisciplinary biology 030306 microbiology Varroidae RNA Honey bee Bees biology.organism_classification Neisseriaceae Gastrointestinal Microbiome 03 medical and health sciences Immune system RNA interference Pollinator Gene expression Animals RNA Interference Varroa Genetic Engineering Symbiosis Functional genomics 030304 developmental biology
Zdroj:	Science. 367:573-576
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.aax9039
Popis:	Inducing immune bee genes Honey bees are prone to parasitism by the Varroa mite, which is a vector for several bee pathogens. However, honey bees are also host to the symbiotic gut bacterium Snodgrassella alvi. Leonard et al. engineered S. alvi to produce double-stranded RNA (dsRNA)—a stimulus for insect RNA interference defense responses—from a plasmid containing two inverted promoters tagged with a fluorescent label (see the Perspective by Paxton). This dsRNA module can be targeted to interfere with specific bee genes as well as crucial viral and mite genes. The authors found that gene expression could be blocked for at least 15 days as the symbionts established in the bees' guts and continuously expressed the dsRNA constructs. S. alvi with specifically targeted plasmids not only suppressed infection with deformed wing virus but also effectively reduced Varroa mite survival. Science , this issue p. 573 ; see also p. 504
Databáze:	OpenAIRE
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