Different intrathyroid expression of intercellular adhesion molecule-1 (ICAM-1) in Hashimoto’s thyroiditis and Graves’ disease: Analysis at mRNA level and association with B7.1 costimulatory molecule
| Autor: | A. M. Riccio, P. Montagna, V. Altrinetti, Giancarlo Torre, C. Salmaso, Giampaola Pesce, N. Fiorino, Marcello Bagnasco |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
endocrine system Pathology medicine.medical_specialty Autoimmune thyroiditis Goiter endocrine system diseases ICAM-1 Endocrinology Diabetes and Metabolism Graves' disease Thyroid Gland Gene Expression Intercellular adhesion In situ hybridization Thyroiditis Endocrinology Thyroid peroxidase medicine Humans RNA Messenger In Situ Hybridization Thyroid Epithelial Cells Thyroid biology business.industry Thyroiditis Autoimmune Middle Aged Alkaline Phosphatase Intercellular Adhesion Molecule-1 Autoimmune thyroiditis B7 Costimulation ICAM-1 Intercellular adhesion Thyroid medicine.disease Immunohistochemistry Molecular biology Graves Disease Costimulation medicine.anatomical_structure B7-1 Antigen biology.protein Female B7 business Goiter Nodular |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1720-8386 0391-4097 |
| DOI: | 10.1007/bf03344004 |
| Popis: | Cultured thyroid epithelial cells can be induced to express intercellular adhesion molecule-1 (ICAM-1, or CD54). However, constitutive follicular expression of ICAM-1 has been reported only in thyroid autoimmunity. We evaluated the expression of ICAM-1 mRNA and protein on thyroid tissue from different autoimmune thyroid diseases, and its relationship with other immunologically relevant surface markers, namely costimulatory molecules of B7 family. Thyroid tissue sections were obtained by surgically removed thyroid glands from 6 patients with Hashimoto's thyroiditis (HT), 6 with Graves' disease (GD) and 3 with multinodular nontoxic goiter. We used in situ hybridization to localize ICAM-1 mRNA, and immunohistochemical analysis by alkaline phosphatase anti-alkaline phosphatase (APAAP) method. We showed a clear hybridization pattern, localized in follicular cells, in sections of glands with HT. The hybridization pattern was far less pronounced in GD: no staining was apparent on follicular cells. These results were strictly consistent with those obtained by means of immunohistochemistry. Moreover, double-staining experiments demonstrated colocalization of ICAM-1 and B7.1 molecules in HT, whereas no B7.1 expression was observed in Graves' or in non-autoimmune thyroid diseases. These data agree with the hypothesis of distinct immunoregulatory phenomena and effector mechanisms in the 2 main autoimmune thyroid diseases. |
| Databáze: | OpenAIRE |
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