A novel qPCR based-method for detection and quantification of three recurrent species of Penicillium isolated from bioaerosols in mold-damaged homes
| Autor: | N. Heutte, Antoine Géry, E. Chosson, Antoine Delanoë, David Garon, J. Bonhomme |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Indoor bioaerosol Fungal contamination Air Microbiology Penicillium brevicompactum medicine.disease_cause Real-Time Polymerase Chain Reaction Microbiology Agar plate 03 medical and health sciences Mold medicine Vaccines DNA Tuberculosis Vaccines Molecular Biology 030304 developmental biology Detection limit Aerosols 0303 health sciences Chromatography biology 030306 microbiology Chemistry Penicillium biology.organism_classification Housing Bioaerosol |
| Zdroj: | Journal of microbiological methods. 186 |
| ISSN: | 1872-8359 |
| Popis: | Fungal contamination of indoor environments can cause respiratory diseases and induce damages to building materials. Among the fungal species found in mold-damaged homes, Penicillium brevicompactum , P. chrysogenum and P. crustosum can be considered as recurrent strains. In this study, we therefore propose a rapid and novel qPCR-based method in order to allow the monitoring of these three fungal species. The method developed allows the quantification of the target DNA of these three Penicillium species with a limit of quantification of 0.01 ng/μL without significant difference with spectrophotometry quantification assay for DNA concentrations between 5 and 100 ng/μL. This technique also enables the rapid detection of these three species in complex mixtures of DNA extracted from 15 bioaerosols collected in mold-damaged homes and previously cultured on agar plate . This new sensitive and specific qPCR technique can thus be easily integrated into bioaerosol studies. |
| Databáze: | OpenAIRE |
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