Specific Cell Behavior of Human Fibroblast onto Carbohydrate Surface Detected by Glycoblotting Films
| Autor: | Ryusuke Kamitani, Norimasa Iwasaki, Noriko Nagahori, Akio Minami, Hideyuki Shimaoka, Tomohiro Onodera, Tokifumi Majima, Kenichi Niikura, Shin-Ichiro Nishimura |
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| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Chemistry Cell growth Glycopolymer Carbohydrates Bioengineering Adhesion Polymer Cellobiose Fibroblasts Carbohydrate Biomaterials chemistry.chemical_compound medicine.anatomical_structure Biochemistry Cell Adhesion Materials Chemistry medicine Biophysics Humans Cell adhesion Fibroblast Cells Cultured Cell Proliferation |
| Zdroj: | Biomacromolecules. 7:2949-2955 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | We synthesized an aminooxyl polymer that is reactive with the reduced end of carbohydrates using our sugar-displaying approach. The carbohydrates were easily immobilized on the polymer film (glycoblotting film) by simple immersion in a in sugar solution through stable oxime bond. The in vitro behaviors of human fibroblasts on the carbohydrate-coated surface were investigated. The adhesion of human fibroblasts on the cellobiose- and cellotriose-coated surfaces was much greater than on the other coated surfaces and the noncoated surface. This result indicated that simple structural differences in carbohydrates induced biological changes in human cells, especially cell adhesion. Our approach provides a high-throughput assay system for carbohydrate-related cell adhesion and proliferation. |
| Databáze: | OpenAIRE |
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