Paenibacillus residui sp. nov., isolated from urban waste compost
| Autor: | Olga C. Nunes, Ivone Vaz-Moreira, Vânia Figueira, Célia M. Manaia, Ana R. Lopes, Rüdiger Pukall, Cathrin Spröer, Peter Schumann |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Peptidoglycan DNA Ribosomal Microbiology Paenibacillus chemistry.chemical_compound Cell Wall Phylogenetics RNA Ribosomal 16S Cluster Analysis Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Spores Bacterial Base Composition biology Fatty Acids Quinones Temperature Sequence Analysis DNA General Medicine Ribosomal RNA Catalase beta-Galactosidase biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques chemistry Oxidoreductases Soil microbiology Bacteria |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Two bacterial strains, MC-246T and MC-247, were isolated from municipal urban waste compost and characterized by a polyphasic approach. Both isolates were Gram-stain-variable, endospore-forming rods that were catalase-, oxidase- and β-galactosidase-positive, and able to grow at 25–50 °C and pH 7.0–9.0, with optimum growth at 37 °C and pH 7. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0; the major respiratory quinone was menaquinone MK-7; the cell wall peptidoglycan was of type A1γ; and the DNA G+C content was 49 mol%. These characteristics, as well as data from 16S RNA gene sequence analysis, showed that these strains were affiliated with the genus Paenibacillus; the type strains of Paenibacillus ginsengarvi and Paenibacillus hodogayensis were among their closest neighbours (T and MC-247 could represent a novel species was supported by the low 16S rRNA gene sequence similarity values shared with other members of the genus Paenibacillus and by the observation of distinct biochemical and physiological traits. Strains MC-246T and MC-247 shared 99.6 % 16S rRNA gene sequence similarity and showed almost identical MALDI-TOF mass spectra, but could be distinguished at the phenotypic and genotypic level. However, DNA–DNA hybridization between strains MC-246T and MC-247 resulted in values above 70 % indicating that both organisms represent a single species, for which the name Paenibacillus residui sp. nov. is proposed; the type strain is MC-246T (=DSM 22072T =CCUG 57263T). |
| Databáze: | OpenAIRE |
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