Introduction of ID2 Enhances Invasiveness in ID2-null Oral Squamous Cell Carcinoma Cells via the SNAIL Axis
| Autor: | Wataru Kumamaru, Akiko Ishikawa, Tomoki Sumida, Yoshihide Mori, Yosuke Kobayashi, Y U Kamata |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research MMP2 Epithelial-Mesenchymal Transition Vimentin Matrix metalloproteinase Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Genetics Humans Neoplasm Invasiveness Epithelial–mesenchymal transition Molecular Biology Transcription factor Cell Proliferation Inhibitor of Differentiation Protein 2 Zinc finger transcription factor Cell growth Cell Differentiation Cadherins Phenotype Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis Cancer research biology.protein Carcinoma Squamous Cell Matrix Metalloproteinase 2 Mouth Neoplasms Snail Family Transcription Factors Signal Transduction Research Article |
| Zdroj: | Cancer genomicsproteomics. 13(6) |
| ISSN: | 1790-6245 |
| Popis: | Aim Inhibitor of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors that generally stimulate cell proliferation and inhibit differentiation. However, the role of ID2 in cancer progression remains ambiguous. Here, we investigated the function of ID2 in ID2-null oral squamous cell carcinoma (OSCC) cells. Materials and methods We introduced an ID2 cDNA construct into ID2-null OSCC cells and compared them with empty-vector-transfected cells in terms of cell proliferation, invasion, and activity and expression of matrix metalloproteinase (MMP). Results ID2 introduction resulted in enhanced malignant phenotypes. The ID2-expressing cells showed increased N-cadherin, vimentin, and E-cadherin expression and epithelial-mesenchymal transition. In addition, cell invasion drastically increased with increased expression and activity of MMP2. Immunoprecipitation revealed a direct interaction between ID2 and zinc finger transcription factor, snail family transcriptional repressor 1 (SNAIL1). Conclusion ID2 expression triggered a malignant phenotype, especially of invasive properties, through the ID2-SNAIL axis. Thus, ID2 represents a potential therapeutic target for OSCC. |
| Databáze: | OpenAIRE |
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