Genetic characterization of the DNA of the bacteriophage φX174 70 S particle
| Autor: | G.A. van Arkel, Peter Weisbeek, J.H. van de Pol |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Genetics
Microbial Sucrose DNA polymerase Base pair viruses DNA polymerase II Cesium Spheroplasts Coliphages Chlorides Virology Centrifugation Density Gradient Escherichia coli Genomic library Alleles Deoxyribonucleases DNA clamp Base Sequence biology Circular bacterial chromosome Nucleic Acid Hybridization Rubidium Molecular biology Molecular Weight DNA Viral Mutation biology.protein Shigella Primer (molecular biology) Biologie Ultracentrifugation In vitro recombination |
| Zdroj: | Virology, 48(2), 456. Elsevier |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(72)90056-6 |
| Popis: | The DNA of the 70 S particle of the bacteriophage φX174 has been characterized. By hybridization of RF II DNA of mutants from five different cistrons with DNA from wild-type 70 S particles it is demonstrated that this latter DNA is heterogeneous. Although the molecular weight is about 20% of the intact φX DNA, the 70 S DNA preparation contains nucleotide sequences from all five cistrons tested. The 70 S DNA turns out to consist of the plus strand type. |
| Databáze: | OpenAIRE |
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