Purification of capsular polysaccharide produced by Haemophilus influenzae type b through a simple, efficient and suitable method for scale-up
| Autor: | Silvia Maria Ferreira Albani, Mickie Takagi, Teresa Cristina Zangirolami, Joaquin Cabrera-Crespo, Rodrigo Barbosa Lima, Martha M. Tanizaki |
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| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Ultrafiltration Centrifugation Bioengineering medicine.disease_cause Polysaccharide Applied Microbiology and Biotechnology Haemophilus influenzae chemistry.chemical_compound Bioreactors medicine Chemical Precipitation Phenol Chelation Ethanol precipitation chemistry.chemical_classification Chromatography Ethanol biology Chemistry Polysaccharides Bacterial Haemophilus influenzae type b biology.organism_classification Bacteria Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 35:1217-1222 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-008-0428-4 |
| Popis: | Haemophilus influenzae type b, an encapsulated bacterium, causes meningitis in infants worldwide. The capsular polysaccharide conjugated to a carrier protein is effective in the prevention of such infections. The traditional purification process of polysaccharide from bacterial cultures for vaccine production is based on several selective precipitations with solvents such as: ethanol, phenol, and cationic detergents. The separations of solid and liquid phases are based on continuous centrifugation in explosion proof installations. The lipopolysaccharides are separated by ultracentrifugation. A simple and efficient method that can easily be scaled-up was developed for purification of polysaccharides. The ethanol precipitation was reduced to only two steps. The phenol treatment was substituted by ultrafiltration and enzymatic digestion. Lipopolysaccharide was removed by ultrafiltration together with addition of detergent and chelating agent. |
| Databáze: | OpenAIRE |
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