Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis
| Autor: | Lanmei Wang, Di Zuo, Weiwei Lv, Yunlong Zhao, Dan-Li Wang, Ajing Liu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Protein subunit Molecular Sequence Data Gene Expression Astacoidea Biology Oogenesis Gene Expression Regulation Enzymologic Arthropod Proteins Complementary DNA Cherax quadricarinatus CDC2 Protein Kinase Genetics Animals Amino Acid Sequence Phylogeny Cyclin-dependent kinase 1 Messenger RNA Base Sequence Sequence Homology Amino Acid urogenital system Kinase Ovary Gene Expression Regulation Developmental Sequence Analysis DNA General Medicine biology.organism_classification Crayfish Molecular biology Protein Subunits Organ Specificity Female |
| Zdroj: | Gene. 515:258-265 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2012.11.082 |
| Popis: | Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1769 bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7 kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus. |
| Databáze: | OpenAIRE |
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