Nitric oxide modulated the expression of DREAM/calsenilin/KChIP3 in inflammatory pain of rats
| Autor: | Ying-Li Song, Yong-Bin Yang, Yu-Rong Li, Hong-Bo Jin, Yong-Liang Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Immunology Pain Inflammation Nitric Oxide Nitroarginine Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Downregulation and upregulation Internal medicine Formaldehyde medicine Immunology and Allergy Animals RNA Messenger Injections Spinal Pain Measurement Morphine business.industry Kv Channel-Interacting Proteins Spinal cord humanities Rats Repressor Proteins Nociception Endocrinology medicine.anatomical_structure chemistry Spinal Cord Anesthesia Calsenilin medicine.symptom business psychological phenomena and processes medicine.drug |
| Zdroj: | Inflammation. 35(6) |
| ISSN: | 1573-2576 |
| Popis: | Downstream regulatory element antagonistic modulator (DREAM) is a critical transcriptional repressor for pain modulation. The role of nitric oxide (NO) plays in modulating DREAM pain pathway in the periphery is unclear. Therefore, we investigated the role of the NO in modulation of the expression of DREAM in formalin-induced rat inflammatory pain models. Male Sprague-Dawley rats were randomly distributed into four groups: the normal group, formalin test group, Nω-nitro-L-arginine (l-NNA) group, and morphine group. One hundred microliters of 2.5 % formalin was injected into the plantar surface of the right hindpaw of rats. l-NNA (40 nmol/L) and morphine (40 nmol/L) were injected intrathecally in the hindpaw before formalin injection. The nociceptive behavioral reaction was recorded. After the formalin test, the expression of DREAM mRNA and protein in the spinal cord of the four groups were measured. The nociceptive reaction induced by injection of formalin exhibited two phases. Morphine and l-NNA significantly decreased pain scores of the second phase. The expression of DREAM was significantly increased in the rat spinal cord after formalin-induced pain. Morphine significantly upregulated the expression of DREAM, and the formalin-induced upregulation was significantly attenuated by l-NNA. NO may play an important role in the DREAM pathway modulation of inflammatory pain. |
| Databáze: | OpenAIRE |
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