The presence of CD55- and/or CD59-deficient erythrocytic populations in patients with rheumatic diseases reflects an immune-mediated bone-marrow derived phenomenon
| Autor: | Michael Samarkos, Evangelos Terpos, John V. Asimakopoulos, Theodoros P. Vassilakopoulos, George Vaiopoulos, Loula Papageorgiou, Dimitrios Christoulas, Anastasios Giakoumis, Olga Kampouropoulou, John Meletis, Maria K. Angelopoulou, Konstantinos Konstantopoulos |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Anemia Hemolytic Erythrocytes Anemia Paroxysmal Hemoglobinuria Paroxysmal Hemoglobinuria chemical and pharmacologic phenomena CD59 030204 cardiovascular system & hematology Biology Autoimmune Diseases Hemoglobins 03 medical and health sciences 0302 clinical medicine Immune system Antigen Clinical Research Rheumatic Diseases medicine Humans Peripheral blood cell Antigens Aged CD55 Antigens complement regulatory proteins General Medicine Middle Aged medicine.disease 3. Good health Complement system medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Female Bone marrow CD55 |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | Background Complement has the potential to provoke severe impairment to host tissues, as shown in autoimmune diseases where complement activation has been associated with diminished CD55 and/or CD59 expression on peripheral blood cell membranes. The aim of this study was to evaluate the presence of CD55- and/or CD59-deficient erythrocytic populations in patients with different rheumatic diseases and to investigate possible correlations with clinical or laboratory parameters. Material/Methods CD55 and CD59 expression was evaluated in erythrocytes of 113 patients with rheumatic diseases, 121 normal individuals, and 10 patients with paroxysmal nocturnal hemoglobinuria (PNH) using the Sephacryl gel microtyping system. Ham and sucrose tests were also performed. Results Interestingly, the majority of patients (104/113, 92%) demonstrated CD55- and/or CD59-deficient erythrocytes: 47 (41.6%) with concomitant deficiency of CD55 and CD59, 50 (44.2%) with isolated deficiency of CD55, and 6 (6.2%) with isolated deficiency of CD59. In normal individuals, only 2 (1%) had concomitant CD55/CD59 negativity and 3 (2%) had isolated CD55 or CD59 deficiency. All PNH patients exhibited simultaneous CD55/CD59 deficiency. Positive Ham and sucrose tests were found only in PNH patients. There was no association between the CD55- and/or CD59-deficient erythrocytes and hemocytopenias or undergoing treatment. However, CD55 expression significantly influenced hemoglobin values (F=6.092, p=0.015). Conclusions This study provides evidence supporting the presence of erythrocytes with CD55 and/or CD59 deficiency in patients with rheumatic diseases. Moreover, CD55 deficiency on red cells influences hemoglobin concentration. Further studies using molecular techniques will clarify the exact pathophysiological mechanisms of this deficiency. |
| Databáze: | OpenAIRE |
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