Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low-density lipoprotein
| Autor: | Bo‑Jie Li, Su‑Su Jiang, Shi‑Yin Long, Xin‑Xin Ding, Tian Tian, Min Zhang, Ying Tian, Chu‑Yao Wang, Yu‑Lin Yuan, Cai‑Ping Zhang, Jin‑Qi Shao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Cell medicine.disease_cause Biochemistry Umbilical vein endothelial dysfunction Cell Line 03 medical and health sciences 0302 clinical medicine Genetics medicine Autophagy Human Umbilical Vein Endothelial Cells Humans lipophagy Endothelial dysfunction Molecular Biology oxidized low-density lipoprotein Chemistry Colocalization Articles medicine.disease Lipid Metabolism Cell biology Lipoproteins LDL Microscopy Electron Oxidative Stress 030104 developmental biology medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis oxidative stress injury Molecular Medicine lipids (amino acids peptides and proteins) Oxidative stress Lipoprotein |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Oxidative stress induces the formation of oxidized low‑density lipoprotein (ox‑LDL), which accelerates the development of atherosclerosis and the rupture of atherosclerotic plaques by promoting lipid accumulation and inhibiting autophagy in vascular cells. Lipophagy is known to be involved in maintaining the balance of neutral lipid metabolism; however, the phenomenon of lipophagy deficiency in ox‑LDL‑treated endothelial cells (ECs) remains to be elucidated. It has been demonstrated that lipid accumulation caused by ox‑LDL inhibits autophagy, which promotes apoptosis in ECs. The aim of the present study was to investigate the association between decreased autophagy and lipid accumulation in ECs treated with ox‑LDL. Electron microscopy demonstrated that the formation of autolipophagosomes was decreased in ox‑LDL‑treated human umbilical vein ECs compared with that in the LDL‑treated group and was accompanied by a decrease in the autophagy‑associated proteins via western blotting analysis. Using laser focal colocalization detection, decreased lipid processing was observed in the lysosomes of ox‑LDL‑treated ECs, which indicated that lipophagy may be attenuated and subsequently result in lipid accumulation in ox‑LDL‑treated ECs. |
| Databáze: | OpenAIRE |
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