Evaluation of the possible protective role of naringenin on gentamicin-induced ototoxicity: A preliminary study

Autor: İlker Koçak, Erol Senturk, Dilek Akakin, E. Aydogan, Ömer Faruk Özer, K. Koroglu, S. Sarac
Přispěvatelé: ŞENTÜRK, EROL
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Naringenin
Otoacoustic Emissions
Spontaneous
Otoacoustic emission
Apoptosis
Pharmacology
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
Ototoxicity
parasitic diseases
Evoked Potentials
Auditory
Brain Stem
In Situ Nick-End Labeling
otorhinolaryngologic diseases
medicine
Animals
A preliminary study-
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
cilt.100
ss.247-253
2017 [Kocak I.
Sarac S.
Aydogan E.
SENTURK E.
AKAKIN D.
KÖROĞLU K.
Ozer O. F.
-Evaluation of the possible protective role of naringenin on gentamicin-induced ototoxicity]
Ear Diseases
Cochlea
TUNEL assay
business.industry
food and beverages
General Medicine
medicine.disease
Anti-Bacterial Agents
Rats
carbohydrates (lipids)
030104 developmental biology
Auditory brainstem response
Otorhinolaryngology
chemistry
Ear
Inner
Anesthesia
Flavanones
Pediatrics
Perinatology and Child Health
Gentamicin
Gentamicins
business
medicine.drug
Zdroj: International Journal of Pediatric Otorhinolaryngology. 100:247-253
ISSN: 0165-5876
DOI: 10.1016/j.ijporl.2017.07.008
Popis: The purpose of this study is to evaluate the possible protective role of naringenin in gentamicin-induced ototoxicity through an audiological, biochemical and histopathological evaluation.This study was conducted on 32 adult male rats that were randomized into 4 groups(control, gentamicin, naringenin + gentamicin, and naringenin). Naringenin was given to the rats via oral gavage in a dose of 50 mg/kg/day during the 14 day study period. Gentamicin was given by the intraperitoneal route in a dose of 120 mg/kg/day. Audiological assessment was performed by the distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) measurements, applied to all rats at the beginning of the study, and also on day 14. Biochemical parameters were calculated on day 14 to evaluate the oxidative and antioxidative status. Their cochleae were removed and examined histopathologically, also on day 14. The cochlea of animals were evaluated with the terminal deoxynucleotidyl transferase-mediated dUTPbiotin nick end labeling (TUNEL) method for apoptosis.On days 14, DPOAE values and ABR thresholds were preserved in group 3(naringenin + gentamicin) when compared with group 2(gentamicin)(p 0.008). The total oxidant status values and oxidative stress index values were significantly higher in group 2(gentamicin) than in other groups (p 0.008). The total antioxidant status value was significantly higher in group 3(naringenin + gentamicin) and group 4(naringenin) than in group 2(gentamicin)(p 0.008). The number of TUNEL positive cells in both the organ of Corti and the stria vascularis were found to be statistically lower in group 3(naringenin + gentamicin) than in group 2(gentamicin)(p 0.05).Our study has demonstrated that the ototoxic effect generated by gentamicin could be ameliorated with the concurrent use of naringenin.
Databáze: OpenAIRE
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