Growth and Autologous Tumor Lysis by Tumor-Infiltrating Lymphocytes from Metastatic Melanoma Expanded in Interleukin-2 or Interleukin-2 plus Interleukin-4
| Autor: | Alexander Fefer, Catherine G. Lindgren, John A. Thompson, C. Higuchi |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cytotoxicity
Immunologic Interleukin 2 Cancer Research Pathology medicine.medical_specialty Adoptive cell transfer medicine.medical_treatment Immunology chemical and pharmacologic phenomena Lymphocytes Tumor-Infiltrating Biopsy Humans Immunology and Allergy Medicine Melanoma Cells Cultured Interleukin 4 Pharmacology medicine.diagnostic_test business.industry Tumor-infiltrating lymphocytes Interleukin hemic and immune systems Immunotherapy medicine.disease Phenotype Interleukin-2 Interleukin-4 business medicine.drug |
| Zdroj: | Journal of Immunotherapy. 14:322-328 |
| ISSN: | 1524-9557 |
| Popis: | Optimal conditions for expanding tumor-infiltrating lymphocytes (TILs) specifically cytotoxic for autologous melanoma for clinical use have not yet been identified. In several small studies, interleukin (IL)-4 was reported to promote the growth of such TILs in IL-2. Given the potential implications for TIL therapy, we attempted to confirm these findings in a larger study. Baseline data were first obtained on the proliferation, immunophenotype, and cytotoxic reactivity to autologous melanoma of TILs cultured in IL-2 alone. Similar studies were performed with TIL cultured concurrently in either IL-2 alone or in a combination of IL-2 and IL-4. TILs were obtained by excisional biopsy of tumors from 52 patients with metastatic malignant melanoma; TILs from 38 patients were expanded in IL-2 (1,000 U/ml). TILs from 19 biopsies were maximally expanded 6- to 24,000-fold (median, 300-fold) over 4-10 weeks. Expansion did not correlate with the weight of, or number of lymphocytes in, the biopsy specimen, or the site of the biopsy (lymph node vs. subcutaneous metastases). During weeks 5-8, TILs from 19 of 25 biopsy specimens lysed autologous melanoma with little or no lysis of allogeneic melanoma. Lysis of autologous tumor was blocked by antibody to class I antigens. Twenty-four TIL specimens were cultured concurrently in IL-2 alone and in IL-2 plus IL-4 and tested for growth and for lysis of autologous and allogeneic melanomas.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|