Affinity monolith chromatography: A review of general principles and applications
| Autor: | Zhao Li, Allegra Pekarek, Elliott Rodriguez, Shiden Azaria, David S. Hage |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
musculoskeletal diseases
Analyte geography Chromatography geography.geographical_feature_category Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry Clinical Biochemistry Proteins Stereoisomerism 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Chromatography Affinity Article 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Affinity chromatography Stationary phase Agarose Monolith 0210 nano-technology |
| Zdroj: | ELECTROPHORESIS. 38:2837-2850 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological-binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose, and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined. |
| Databáze: | OpenAIRE |
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