Demonstration of a heterogeneous transcription pattern of thyroglobulin mRNA in human thyroid tissues
| Autor: | F. Bertaux, Philippe Fragu, Yves Malthièry, Michéle Noel |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adenoma
Transcription Genetic endocrine system diseases RNA Splicing medicine.medical_treatment Restriction Mapping Thyroid Gland Biophysics Biology Polymerase Chain Reaction Thyroglobulin Biochemistry Thyroid carcinoma medicine Humans RNA Messenger Thyroid Neoplasms Northern blot Molecular Biology Messenger RNA Epithelioma Carcinoma Alternative splicing Thyroid Nucleic Acid Hybridization Cell Biology medicine.disease Molecular biology Blotting Southern medicine.anatomical_structure Chromosome Deletion Oligonucleotide Probes |
| Zdroj: | Biochemical and Biophysical Research Communications. 178:586-592 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(91)90148-z |
| Popis: | Previous reports on human thyroglobulin (hTg) modifications in thyroid carcinomas prompted us to study hTg mRNA in thyroid adenomas and carcinomas. The quantification of hTg mRNA showed a decrease in its levels of expression in both pathological conditions which differed by a factor of 2 between adenomas and carcinomas. Furthermore, PCR was used to analyse the characteristics of hTg mRNA by amplifying 4 regions of the hTg mRNA. When applied to 2 normal, 17 benign and 13 malignant pathological tissue specimens, PCR showed no modification in the size of Tg mRNA. However, abnormal sized cDNAs appeared in all tissues with no distinction between the pathologies; the Restriction Fragment Length Polymorphism study of these cDNAs suggests the existence of alternate splicing patterns in thyroglobulin mRNAs. |
| Databáze: | OpenAIRE |
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