Calibration, Specificity and Trueness of a Postheparin Plasma Lipoprotein Lipase Assay
| Autor: | Per Hyltoft Petersen, Henning Beck-Nielsen, Finn L. Henriksen, Mogens Hørder |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipoprotein lipase activity
Calibration curve Coefficient of variation Clinical Biochemistry Sensitivity and Specificity Postheparin plasma Calibration Animals Humans Lipase Analytical within-run variation Observer Variation Calibration system Reproducibility Lipoprotein lipase Chromatography biology Heparin Chemistry Biochemistry (medical) Analytical between-run variation General Medicine Lipoprotein Lipase Biochemistry biology.protein Cattle |
| Zdroj: | Henriksen, F L, Petersen, P H, Beck-Nielsen, H & Hørder, M 2001, ' Calibration, specificity and trueness of a postheparin plasma lipoprotein lipase assay ', Clinical Chemistry and Laboratory Medicine, vol. 39, no. 3, pp. 263-269 . https://doi.org/10.1515/CCLM.2001.041 |
| ISSN: | 1434-6621 |
| DOI: | 10.1515/cclm.2001.041 |
| Popis: | Measurement of lipoprotein lipase activity in postheparin plasma is generally accompanied by moderate within-run variation CVW-R (B-R (5-25%). A calibration system was introduced in order to improve the reproducibility of measurements and to compare lipoprotein lipase activities from different days. Every day a calibration curve for lipoprotein lipase activity was constructed. Fifteen calibration curves designed over 2 years, show linearity over the whole biological spectrum and a considerable reduction of between-run variation in lipoprotein lipase activity, from 42% to 5.3% as estimated from two control postheparin plasma samples. The lipoprotein lipase calibration system is an easy and very cheap arrangement, which makes it possible to compare lipoprotein lipase activities achieved over years. When the lipoprotein lipase control values are compared with reference lipoprotein lipase samples determined in other lipase laboratories, the calibration-control system becomes an important tool for reducing analytical bias. The article reviews the original analytical criteria of catalytic measurement of lipoprotein lipase activity and describes the implementation of the calibration-control system. We describe a model for reduction of the analytical variability in the measurement of lipoprotein lipase activity. Other standardization efforts need to be made in the future, especially to define the reference material for calibration. |
| Databáze: | OpenAIRE |
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