Morphological and Inter Simple Sequence Repeat (ISSR) markers analyses of Corynespora cassiicola isolates from rubber plantations in Malaysia
| Autor: | Jugah Kadir, E. Sunderasan, Mohd Puad Abdullah, Adam Malik, Suhaimi Napis, Nguyen Anh Nghia |
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| Rok vydání: | 2008 |
| Předmět: |
Veterinary medicine
Veterinary (miscellaneous) Applied Microbiology and Biotechnology Microbiology Polymerase Chain Reaction law.invention Ascomycota law Phylogenetics Botany Cluster Analysis Corynespora cassiicola Polymerase chain reaction Phylogeny Repetitive Sequences Nucleic Acid Electrophoresis Agar Gel biology Geography Dendrogram Fungal genetics UPGMA Malaysia Spores Fungal biology.organism_classification Hevea Hevea brasiliensis Agronomy and Crop Science |
| Zdroj: | Mycopathologia. 166(4) |
| ISSN: | 0301-486X |
| Popis: | Morphological features and Inter Simple Sequence Repeat (ISSR) polymorphism were employed to analyse 21 Corynespora cassiicola isolates obtained from a number of Hevea clones grown in rubber plantations in Malaysia. The C. cassiicola isolates used in this study were collected from several states in Malaysia from 1998 to 2005. The morphology of the isolates was characteristic of that previously described for C. cassiicola. Variations in colony and conidial morphology were observed not only among isolates but also within a single isolate with no inclination to either clonal or geographical origin of the isolates. ISSR analysis delineated the isolates into two distinct clusters. The dendrogram created from UPGMA analysis based on Nei and Li’s coefficient (calculated from the binary matrix data of 106 amplified DNA bands generated from 8 ISSR primers) showed that cluster 1 encompasses 12 isolates from the states of Johor and Selangor (this cluster was further split into 2 sub clusters (1A, 1B), sub cluster 1B consists of a unique isolate, CKT05D); while cluster 2 comprises of 9 isolates that were obtained from the other states. Detached leaf assay performed on selected Hevea clones showed that the pathogenicity of representative isolates from cluster 1 (with the exception of CKT05D) resembled that of race 1; and isolates in cluster 2 showed pathogenicity similar to race 2 of the fungus that was previously identified in Malaysia. The isolate CKT05D from sub cluster 1B showed pathogenicity dissimilar to either race 1 or race 2. |
| Databáze: | OpenAIRE |
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