Differences in Env and Gag protein expression patterns and epitope availability in feline immunodeficiency virus infected PBMC compared to infected and transfected feline model cell lines
| Autor: | Isaura Christiaens, Sebastiaan Theuns, Hans Nauwynck, Lowiese Desmarets, Delphine D. Acar, Chris K. Grant, Inge D. M. Roukaerts, Sebastiaan Van Bockstael |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Feline immunodeficiency virus Glycosylation viruses Gene Expression Gene Products gag Immunodeficiency Virus Feline Antibodies Viral Epitope Cell Line 03 medical and health sciences Epitopes Antigen Virology Feline Acquired Immunodeficiency Syndrome Animals CD134 Protein Interaction Domains and Motifs Amino Acid Sequence Cells Cultured Viral matrix protein biology Cell Membrane virus diseases Antibodies Monoclonal Gene Products env biochemical phenomena metabolism and nutrition Group-specific antigen biology.organism_classification Molecular biology 030104 developmental biology Infectious Diseases Virion assembly Humoral immunity Cats Leukocytes Mononuclear Protein Multimerization Protein Binding |
| Zdroj: | Virus research. 227 |
| ISSN: | 1872-7492 |
| Popis: | Env and Gag are key components of the FIV virion that are targeted to the plasma membrane for virion assembly. They are both important stimulators and targets of anti-FIV immunity. To investigate and compare the expression pattern and antigenic changes of Gag and Env in various research models, infected PBMC (the natural FIV host cells) and GFox, and transfected CrFK were stained over time with various Env and Gag specific MAbs. In FIV infected GFox and PBMC, Env showed changes in epitope availability for antibody binding during processing and trafficking, which was not seen in transfected CrFK. Interestingly, epitopes exposed on intracellular Env and Env present on the plasma membrane of CrFK and GFox seem to be hidden on plasma membrane expressed Env of FIV infected PBMC. A kinetic follow up of Gag and Env expression showed a polarization of both Gag and Env expression to specific sites at the plasma membrane of PBMC, but not in other cell lines. In conclusion, mature trimeric cell surface expressed Env might be antigenically distinct from intracellular monomeric Env in PBMC and might possibly be unrecognizable by feline humoral immunity. In addition, Env expression is restricted to a small area on the plasma membrane and co-localizes with a large moiety of Gag, which may represent a preferred FIV budding site, or initiation of virological synapses with direct cell-to-cell virus transmission. |
| Databáze: | OpenAIRE |
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