Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models
| Autor: | Edson Lucas dos Santos, Alexeia Barufatti, Luiza Flávia Veiga Francisco, Lucilene Finoto Viana, Syla Maria Farias Ferraz Klafke, Débora da Silva Baldivia, Bruno do Amaral Crispim, Kelly Mari Pires de Oliveira, Pamella Fukuda de Castilho |
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| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Hamster metals TP1-1185 010501 environmental sciences Toxicology 01 natural sciences Article Ames test 03 medical and health sciences comet assay micronucleus Cytotoxicity 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Chemical Health and Safety Chemistry Chemical technology Chinese hamster ovary cell CHO cells Molecular biology Comet assay Micronucleus test Microsome cytotoxicity Micronucleus |
| Zdroj: | Toxics Volume 9 Issue 7 Toxics, Vol 9, Iss 153, p 153 (2021) |
| ISSN: | 2305-6304 |
| Popis: | The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater. |
| Databáze: | OpenAIRE |
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