[Modified HPLC method of determination of the valproic acid in biological fluids]
| Autor: | A. A. Dutov, V I Letunov, D. A. Nikitin, P P Tereshkov, O N Konovalova |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Sorbent Acetonitriles Adolescent High-performance liquid chromatography Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology 2-Propanol chemistry.chemical_compound Acetone Humans Derivatization Acetonitrile Triethylamine Chromatography High Pressure Liquid Chromatography Epilepsy Valproic Acid Extraction (chemistry) Heptanoic acid Water General Medicine Middle Aged chemistry Anticonvulsants Female |
| Zdroj: | Biomeditsinskaia khimiia. 59(5) |
| ISSN: | 2310-6972 |
| Popis: | Proposed modified HPLC method for determination of valproic acid in biological fluids. Created solid-phase extraction of valproic and heptanoic acids (internal standard, IS) on the cartridges packed hyper cross-linked polystyrene which maintain some tens extractions without losses of efficiency. Carboxylic acids are derivative with 1-(bromoacetyl)pyrene in acetone at presence of triethylamine. Chromatographic separation of derivatives is performed on Chromolith Performance RP-18e columns, which packed unique monolithic sorbent. UV detection at 360 nm. Mobile phase acetonitrile – water (90:10, v/v) plus 1% isopropanol, speed flow 2000 microL/min, pressure 21 bar. Complete chromatographic cycle less than 3 minutes. Yield of IS and valproic acid (extraction plus derivatization) was 101-106%. Sensitivity (limit detection) was near 1 ng for valproic and near 0.6 ng for heptanoic acid during signal/noise ratio = 3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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