Cloning of hybridoma cell lines by limiting dilution

Autor:	Miyoko Takahashi, Steven A. Fuller, John G.R. Hurrell
Rok vydání:	2008
Předmět:	Cloning clone (Java method) Hybridomas biology Cloning Organism Antibodies Monoclonal General Medicine Molecular biology Dilution Antibody production stomatognathic diseases Hybridoma cell Mice Limiting dilution biology.protein Animals Antibody Cloning procedures
Zdroj:	Current Protocols in Molecular Biology
ISSN:	1934-3647
Popis:	Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b68b0d975b5090b124b74c66d3bc401 https://pubmed.ncbi.nlm.nih.gov/18265080 Zobrazit plný text záznamu Plný text