Use of random amplified polymorphic DNA to identify several novel markers for sex identification in the crested serpent eagle and crested goshawk
| Autor: | Shih-Torng Ding, H. A. Hsu, L. M. Wang, Pei-Hwa Wang, P. I. Lin, C.H. Chang, F. T. Chan, M.C. Chao, Hsiao-Wei Yuan |
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| Rok vydání: | 2009 |
| Předmět: |
Genetic Markers
Male Eagle Sex Determination Analysis Eagles Molecular Sequence Data EcoRI Spilornis cheela Food Animals biology.animal Hoya Animals Small Animals Genetics Sex Characteristics Base Sequence biology Equine Nucleic acid sequence biology.organism_classification DNA Fingerprinting Hawks Random Amplified Polymorphic DNA Technique RAPD DNA-Binding Proteins Genetic marker biology.protein Female Animal Science and Zoology Primer (molecular biology) Sequence Alignment |
| Zdroj: | Theriogenology. 72:755-764 |
| ISSN: | 0093-691X |
| Popis: | The crested serpent eagle (Spilornis cheela hoya) has no distinct sexual dimorphic traits. In the current study, we report the results of an EE0.6 (EcoRI 0.6-kb fragment) sequence applied to S. cheela hoya and a novel random amplified polymorphic DNA (RAPD) marker that can be used to sex individuals within the species S. cheela hoya and Accipiter trivigatus formosae (crested goshawk). We used sex-specific primers for the avian CHD1 (chromo-helicase-DNA-binding 1) gene and the EE0.6 sequence in PCR assays to determine sex. In addition, 120 random primers were used for RAPD fingerprinting to search for novel sex-specific fragments of S. cheela hoya. The OPBB08 random primer generated a 1241-bp sex-specific fragment in all female S. cheela hoya. From the nucleotide sequence, PCR primers were designed to amplify 553-, 895-, and 194-bp sex-specific fragments present in all female S. cheela hoya. One of these primer pairs (ScBB08-7F/R) also amplified a male/female common fragment that can be used as an internal control (543 bp). Moreover, one of the primer pairs (ScBB08-5aF/5bR) could be used to identify genders of A. trivigatus formosae. In conclusion, we identified novel sex-specific DNA markers of S. cheela hoya and A. trivigatus formosae that can be used for rapid and accurate sex identification. |
| Databáze: | OpenAIRE |
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