Involvement of the L-cysteine biosynthetic pathway in azide-induced mutagenesis in Salmonella typhimurium
| Autor: | Marcin Filutowicz, Zygmunt Cieśla, T. Kłopotowski |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Salmonella typhimurium
endocrine system Azides Health Toxicology and Mutagenesis Mutant Mutagen Biology medicine.disease_cause chemistry.chemical_compound Transduction Genetic Genetics medicine Cysteine Molecular Biology Escherichia coli chemistry.chemical_classification Mutation fungi Mutagenesis food and beverages Drug Resistance Microbial Enzyme chemistry Biochemistry Genes Azide Mutagens |
| Zdroj: | Mutation research. 70(3) |
| ISSN: | 0027-5107 |
| Popis: | L-Cystine and L-cysteine specifically reverse the mutagenic action of azide in Salmonella typhimurium and Escherichia coli. To establish whether the L-cysteine biosynthetic pathway is involved in azide-induced mutagenesis, several derivatives of a mutagen tester-strain of S. typhimurium bearing mutations in different cys genes were isolated. No mutagenic effect of azide was observed in a strain carrying mutation in the cysE gene, unless the incubation medium was supplemented with exogenous O-acetylserine. Our of 16 cysK mutants 14 were mutagenized by azide very poorly or not at all. These results indicate that the activity of O-acetylserine sulfhydrylase A, and the availability of O-acetylserine, one of the two co-substrates of the enzyme, are essential for the mutagenic action of azide in S. typhimurium. |
| Databáze: | OpenAIRE |
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