Leucobacter muris sp. nov., isolated from the nose of a laboratory mouse
| Autor: | Martin Sager, W. Peter M. Benten, Eva Engelhardt, Peter Schumann, Laurentiu Benga, Susanne Verbarg, Cathrin Spröer, Boyke Bunk |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial food.ingredient Biology Nose 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Mice food Cell Wall Animals Laboratory Germany RNA Ribosomal 16S Animals Gene Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Alanine chemistry.chemical_classification Base Composition Phylogenetic tree Fatty Acids Laboratory mouse Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA 16S ribosomal RNA Amino acid Bacterial Typing Techniques Actinobacteria genomic DNA 030104 developmental biology chemistry Glycolipids Leucobacter |
| Zdroj: | International journal of systematic and evolutionary microbiology. 69(7) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12T, was isolated from the nose of a laboratory mouse in Dusseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter . Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12T and the type strains of the species Leucobacter chironomi DSM 19883T (99.5 %), followed by Leucobacter celer subsp. astrifaciens CBX151T (97.6 %), Leucobacter celer subsp. celer NAL101T (97.5 %), ‘ Leucobacter kyeonggiensis ’ F3-P9 (97.5 %), Leucobacter zeae CC-MF41T (97.3 %), Leucobacter chromiiresistens JG31T (97.1 %), Leucobacter triazinivorans JW-1T (97.1 %), Leucobacter corticis 2 C-7T (97.0 %) and Leucobacter aridicolis CIP108388T (97.0 %). DNA–DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12T from the type strain of L. chironomi , revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12T was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12T represents a novel species within the genus Leucobacter , family Microbacteriaceae , for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12T (=DSM 101948T=CCM 8761T). |
| Databáze: | OpenAIRE |
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