Adhesive protein expression on endothelial cells after contact in vitro with polyethylene terephthalate coated with pyrolytic carbon
| Autor: | Elisabetta Cenni, Donatella Granchi, Lucia Savarino, D. Cavedagna, Arturo Pizzoferrato, Carla Renata Arciola, S. Stea, A. Di Leo, Gabriela Ciapetti |
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| Rok vydání: | 1995 |
| Předmět: |
Materials science
Endothelium Biophysics Antigens Differentiation Myelomonocytic Vascular Cell Adhesion Molecule-1 Biocompatible Materials Bioengineering In Vitro Techniques Umbilical vein Flow cytometry Biomaterials Blood vessel prosthesis Materials Testing Cell Adhesion medicine Humans Cell adhesion Cells Cultured medicine.diagnostic_test Polyethylene Terephthalates Adhesion Intercellular Adhesion Molecule-1 Molecular biology Carbon Blood Vessel Prosthesis Platelet Endothelial Cell Adhesion Molecule-1 Endothelial stem cell Kinetics medicine.anatomical_structure Biochemistry Mechanics of Materials Cell culture cardiovascular system Ceramics and Composites Endothelium Vascular E-Selectin Cell Adhesion Molecules |
| Zdroj: | Biomaterials. 16:1223-1227 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/0142-9612(95)98128-2 |
| Popis: | This research aims at evaluating the expression of some adhesive proteins on endothelial cell surface after contact with polyethylene terephthalate coated with pyrolytic carbon (PET + PC). Twenty-two different cultures of human umbilical vein endothelial cells (HUVECs) were put in contact with PET + PC. Both HUVECs grown without the biomaterial and HUVECs incubated with endotoxin were used as control. After 24 h, platelet endothelial cell adhesion molecule-1 (PECAM-1), endothelial leucocyte adhesion molecule-1 (ELAM-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were evaluated on the cells by monoclonal antibodies and flow cytometry. In agreement with the literature, after 24 h the culture incubated with endotoxin determined a significant increase in the percentage of positive cells for ELAM-1, a significant increase in fluorescence intensity for ICAM-1, and a significant increase in the percentage of positive cells and fluorescence intensity for VCAM-1. After 24 h of culture with PET + PC, no significant variations in the antigens examined were observed. This demonstrates that such material does not activate in vitro the proteins involved in the adhesion between leucocytes and endothelium or in the adhesion between endothelial cells themselves. |
| Databáze: | OpenAIRE |
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