Detailed mapping and loss of heterozygosity analysis suggests a suppressor locus involved in sporadic breast cancer within a distal region of chromosome band 17p13.3
| Autor: | Gavin R M White, Daniel S. Liscia, Graham Casey, Maria Stack, Tiziania Venesio, David Jones, David Crichton, Erika L D Mitchell, Jenny Varley, Mauro Santibanez-Koref, Jim Heighway |
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| Rok vydání: | 1995 |
| Předmět: |
Yeast artificial chromosome
Heterozygote Molecular Sequence Data Breast Neoplasms Locus (genetics) Biology Loss of heterozygosity Gene mapping Chromosome regions Genetics Humans Genes Tumor Suppressor Chromosomes Artificial Yeast Molecular Biology In Situ Hybridization Fluorescence Genetics (clinical) Base Sequence Contig Chromosome Mapping General Medicine Chromosome Banding Chromosome Band Genetic marker Female Chromosome Deletion Chromosomes Human Pair 17 |
| Zdroj: | Human Molecular Genetics. 4:2047-2055 |
| ISSN: | 1460-2083 0964-6906 |
| Popis: | The chromosome region 17p13.3 is thought to encode a tumour suppressor gene involved in sporadic breast cancer and other malignancies. Physical ordering of markers has been carried out by a series of multicolour fluorescent in situ hybridisation (FISH) experiments, using isolated yeast artificial chromosomes (YACs) and cosmids. Eight polymorphic markers ordered within this new physical map and one external marker were used to investigate the pattern of loss of heterozygosity in a panel of 40 sporadic breast tumour patients. The data revealed a region of high loss (60%) within distal 17p13.3, defined by markers D17S926, D17S695 and D17S849 which mapped close together. A contig of YACs was constructed physically linking these three markers. |
| Databáze: | OpenAIRE |
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