A birth from the transfer of a single vitrified-warmed blastocyst using intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient
| Autor: | C. Nishinaka, Yukiko Nakajo, Hiroyuki Abe, Hiromitsu Hattori, Yasuhisa Araki, Takayuki Ishikawa, T. Kyoya, Koichi Kyono |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment Oocyte Retrieval Ovarian hyperstimulation syndrome Biology Intracytoplasmic sperm injection Andrology Mice Human fertilization Microscopy Electron Transmission Ovulation Induction Pregnancy medicine Animals Humans Sperm Injections Intracytoplasmic Blastocyst Calcimycin Infertility Male reproductive and urinary physiology Globozoospermia Cryopreservation Sperm-Ovum Interactions Gynecology Ionophores Cesarean Section urogenital system Obstetrics and Gynecology Oocyte activation Embryo Transfer medicine.disease Spermatozoa Sperm Embryo transfer medicine.anatomical_structure Reproductive Medicine embryonic structures Oocytes Biological Assay Calcium Female Live Birth |
| Zdroj: | Fertility and Sterility. 91:931.e7-931.e11 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2008.10.002 |
| Popis: | Objective To present the effectiveness of diagnostic heterologous intracytoplasmic sperm injection (ICSI), mouse oocyte activation test (MOAT), and ICSI combined with assisted oocyte activation (AOA) in a globozoospermic patient. Design A case report. Setting A private IVF center, Japan. Patient(s) A patient with globozoospermia. Intervention(s) MOAT in a mouse and ICSI combined with AOA in a human. Main Outcome Measure(s) Ultrastructure, MOAT, fertilization, and pregnancy. Result(s) The transmission electron micrographs showed 100% round-headed spermatozoa lacking an acrosome. MOAT showed that the fertilization rate was 68.4% (13/19) when AOA was used but 0% (0/19) when AOA was not used. After the diagnosis of globozoospermia and sperm-related activation deficiency, 17 human mature oocytes were activated with calcium ionophore after ICSI was performed. The fertilization rate was 88.2% (15/17), and 11 blastocysts were cryopreserved using the vitrification method to prevent severe ovarian hyperstimulation syndrome. A single vitrified-warmed blastocyst was transferred. A gestational sac with fetal heart movements was recognized, and a healthy boy weighing 3180 g was born at 40 weeks of gestation by cesarean section without any congenital abnormality. Conclusion(s) MOAT allows discrimination between sperm- and oocyte-related fertilization failures and shows the effectiveness of AOA. |
| Databáze: | OpenAIRE |
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