Use of PCR in resolving diagnostic difficulties potentially caused by genetic variation of hepatitis B virus
| Autor: | K Hino, P Molyneaux, B C Dow, F. J. Van Deursen, P Yates, D Wyatt, L A Wallace, William F. Carman |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Hepatitis B virus HBsAg Fluorescent Antibody Technique Biology Transfection medicine.disease_cause Polymerase Chain Reaction Pathology and Forensic Medicine Antigen Orthohepadnavirus medicine Humans Amino Acid Sequence Cloning Molecular Diagnostic Errors Hepatitis B Antibodies Hepatitis Hepatitis B Surface Antigens virus diseases General Medicine Hepatitis B biology.organism_classification medicine.disease Virology digestive system diseases Hepadnaviridae HBeAg DNA Viral Immunology Research Article |
| Zdroj: | Journal of Clinical Pathology. 51:149-153 |
| ISSN: | 0021-9746 |
| Popis: | AIMS: To assess the relevance of genetic variants of hepatitis B virus (HBV) and to demonstrate the usefulness of the polymerase chain reaction (PCR) in cases of HBV diagnostic difficulty. METHODS: Five serum samples from patients that presented diagnostic difficulty in routine laboratories were sent to a research laboratory for PCR, and if appropriate, S gene sequencing, in vitro expression, and antigenic analysis. RESULTS: The demonstration of HBV in serum by PCR allowed a definitive diagnosis of current infection. One serum sample with poor reactivity in a diagnostic assay had a minor hepatitis B surface antigen (HBsAg) variant and another with very poor reactivity had multiple variants of HBsAg. Transient HBsAg reactivity was observed in a recently vaccinated patient. A hepatitis Be antigen (HBeAg) false positive reaction was noted in a patient from a well defined risk group for HBV. One patient who was strongly HBsAg/HBeAg positive, but anti-hepatitis B core antibody negative, was viraemic. CONCLUSIONS: PCR may become the gold standard for the diagnosis of current HBV infection. HBV variants are responsible for a proportion of diagnostically difficult cases. Modification of commercial assays is necessary to increase the sensitivity of detection of such variants. |
| Databáze: | OpenAIRE |
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