Postnatal Nitric Oxide Inhibition Modifies Neurotensin Effect on ATPase Activity
| Autor: | Anabel Álvarez-Juliá, María Graciela López Ordieres, Georgina Rodríguez de Lores Arnaiz, Alma K. Kemmling |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty NITRIC OXIDE SYNTHASE CIENCIAS MÉDICAS Y DE LA SALUD Ciencias de la Salud Peptide Biology Nitric Oxide Biochemistry Nitric oxide Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine medicine Animals Neurotensin receptor Ouabain Neurotransmitter Neurotensin Cerebral Cortex chemistry.chemical_classification NA+ K+-ATPASE ACTIVITY General Medicine Enzyme assay Rats NEUROTENSIN Otras Ciencias de la Salud Nitric oxide synthase NG-Nitroarginine Methyl Ester Membrane Endocrinology Animals Newborn chemistry [3H]-OUABAIN BINDING Exploratory Behavior biology.protein Female Nitric Oxide Synthase Sodium-Potassium-Exchanging ATPase NITRIC OXIDE Synaptosomes |
| Zdroj: | Neurochemical Research. 36:2278-2286 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-011-0552-9 |
| Popis: | We have previously showed that peptide neurotensin inhibits neuronal Na+, K+-ATPase activity, an effect which involves high affinity neurotensin receptor. Nitric oxide (NO) acts as a neurotransmitter or as a neuromodulator when it is synthesized by neuronal nitric oxide synthase. Neurotensin effect on Na+, K+-ATPase activity was evaluated in cortical synaptosomal membranes isolated from rats injected at 3, 4 and 5 postnatal days with saline (control) or N (ω)-nitro-l-arginine methyl esther (L-NAME), a nitric oxide synthase inhibitor. Assays were carried out at two stages: juvenile (35 days) and adult (56 days) ages. In an open field task, results recorded in juvenile rats markedly differed from those obtained in adult rats. The presence of neurotensin at 3.5 × 10-8-3.5 × 10-6M concentration decreased 16-34% Na+, K +-ATPase activity in membranes purified from control animals. At variance, the peptide failed to alter this enzyme activity in membranes obtained after L-NAME treatment. After administration of L-NAME, [3H]-ouabain binding to membranes isolated from adult male rats decreased 64% in the presence of 1.0 × 10-6M neurotensin, a peptide concentration which only slightly decreased binding to membranes isolated from juvenile rats. It is postulated that early postnatal NO dysfunction may exert a permanent change in neurotensin system that influence later Na+, K +-ATPase response to neurotensin. Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Alvarez Juliá, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Kemmling, Alma Karen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina |
| Databáze: | OpenAIRE |
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