Structures of human deoxycytidine kinase product complexes
| Autor: | Erika V. Soriano, Steven E. Ealick, Valerie C. Clark |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Stereochemistry Protein Conformation Crystallography X-Ray Uridine Diphosphate Substrate Specificity chemistry.chemical_compound Structure-Activity Relationship Structural Biology Deoxycytidine Kinase Structure–activity relationship Transferase Humans Nucleotide salvage Substrate (chemistry) General Medicine Deoxycytidine monophosphate Deoxycytidine kinase Deoxycytidine Monophosphate Recombinant Proteins carbohydrates (lipids) Uridine diphosphate chemistry Biochemistry Multiprotein Complexes Nucleoside |
| Zdroj: | Acta crystallographica. Section D, Biological crystallography. 63(Pt 12) |
| ISSN: | 0907-4449 |
| Popis: | Human deoxycytidine kinase (dCK) is involved in the nucleotide-biosynthesis salvage pathway and has also been shown to phosphorylate several antitumor and antiviral prodrugs. The structures of dCK alone and the dead-end complex of dCK with substrate nucleoside and product ADP or UDP have previously been reported; however, there is currently no structure available for a substrate or product complex. Here, the structures of dCK complexes with the products dCMP, UDP and Mg2+ ion, and with dAMP, UDP and Mg2+ ion are reported. Structural comparisons show that the product complexes with UDP and a dead-end complex with substrate and UDP have similar active-site conformations. |
| Databáze: | OpenAIRE |
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