Investigation of toxicological effects of amorphous silica nanostructures with amine-functionalized surfaces on Vero cells
| Autor: | Denice Schulz Vicentini, Emeline Ferrari, Carmen Simioni, Renata Gonçalves, Luciane Cristina Ouriques, Martina Garcia de Cezar, Rodrigo Costa Puerari, William Gerson Matias |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
Health Toxicology and Mutagenesis 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences 01 natural sciences Malondialdehyde Chlorocebus aethiops Zeta potential Animals Environmental Chemistry Amines Fourier transform infrared spectroscopy Cytotoxicity Vero Cells 0105 earth and related environmental sciences Chemistry Public Health Environmental and Occupational Health General Medicine General Chemistry Silicon Dioxide Pollution Mitochondria Nanostructures 020801 environmental engineering Amorphous solid Nanotoxicology Transmission electron microscopy Vero cell Biological Assay Amine gas treating Nuclear chemistry |
| Zdroj: | Chemosphere. 214:679-687 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2018.09.165 |
| Popis: | Amorphous silica (SiO2) nanostructures are described in the literature as having low toxicity and are widely used in many industrial products. However, surface modifications, such as amine-functionalization, can result in increased cytotoxicity. In this study, amorphous SiO2 nanostructures (SiO2 NS) were synthesized and amine-functionalized with two different amine molecules: primary (SiO2 NS@1) and tri-amine (SiO2 NS@3). The materials were characterized by transmission electron microscopy (TEM), zeta potential (ZP), effective diameter (ED) and surface area measurements, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The toxicity of the three SiO2 NS samples toward Vero cells was evaluated. According to the methyl thiazolyl tetrazolium (MTT) assay, the IC50,24h was 1.477 ± 0.12 g L−1 for SiO2 NS, 0.254 ± 0.07 g L−1 for SiO2 NS@1 and 0.117 ± 0.05 g L−1 for SiO2 NS@3. The order of cytotoxicity was SiO2 NS@3 > SiO2 NS@1 » SiO2 NS. There was an increase in malondialdehyde (MDA) levels and ROS productions in the cells exposed to all three materials. Also, TEM images showed damage on the mitochondria and rough endoplasmic reticulum. |
| Databáze: | OpenAIRE |
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