Effects of Pulse Interval and Dosing Flux on Cells Varying the Relative Velocity of Micro Droplets and Culture Solution
| Autor: | Qi Mei, Dechun Ba, Wang Zhanwei, Ming Hao, Jiuxin Ning, Kun Liu, Yaoshuai Ba, Chen Shulei, Dongyang Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Bioengineering 02 engineering and technology lcsh:Chemical technology lcsh:Chemistry Rhodamine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine cell dosing Chemical Engineering (miscellaneous) lcsh:TP1-1185 Diffusion (business) microdroplet Polydimethylsiloxane Pulse (signal processing) Process Chemistry and Technology convective diffusion Relative velocity 021001 nanoscience & nanotechnology Membrane lcsh:QD1-999 chemistry Diffusion process numerical simulation interdiffusion 0210 nano-technology Mass fraction 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Processes, Vol 6, Iss 8, p 119 (2018) Processes Volume 6 Issue 8 |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr6080119 |
| Popis: | Microdroplet dosing to cell on a chip could meet the demand of narrow diffusion distance, controllable pulse dosing and less impact to cells. In this work, we studied the diffusion process of microdroplet cell pulse dosing in the three-layer sandwich structure of PDMS (polydimethylsiloxane)/PCTE (polycarbonate) microporous membrane/PDMS chip. The mathematical model is established to solve the diffusion process and the process of rhodamine transfer to micro-traps is simulated. The rhodamine mass fraction distribution, pressure field and velocity field around the microdroplet and cell surfaces are analyzed for further study of interdiffusion and convective diffusion effect. The cell pulse dosing time and drug delivery efficiency could be controlled by adjusting microdroplet and culture solution velocity without impairing cells at micro-traps. Furthermore, the accuracy and controllability of the cell dosing pulse time and maximum drug mass fraction on cell surfaces are achieved and the drug effect on cells could be analyzed more precisely especially for neuron cell dosing. |
| Databáze: | OpenAIRE |
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