Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest
| Autor: | Danilo Simonini Teixeira, Maria de Fátima Ferreira-da-Cruz, Cláudio Tadeu Daniel-Ribeiro, Ricardo Lourenço-de-Oliveira, Aline Rosa Lavigne Mello, Edmilson Moutinho dos Santos, Anielle de Pina-Costa, Filipe Vieira Santos de Abreu, Larissa Rodrigues Gomes, Cesare Bianco-Junior, Patrícia Brasil |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Entomology medicine.medical_specialty Plasmodium lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Non-human-primates 030231 tropical medicine Zoology Blood clot lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Atlantic forest lcsh:RC109-216 Alouatta guariba clamitans Plasmodium species biology Yellow fever medicine.disease biology.organism_classification 030104 developmental biology Infectious Diseases Parasitology Tropical medicine Atlantic Forest |
| Zdroj: | Malaria Journal, Vol 17, Iss 1, Pp 1-5 (2018) |
| ISSN: | 1475-2875 |
| Popis: | Background Zoonotic infections with epidemic potential, as non-human primate malaria and yellow fever (YF), can overlap geographically. Optimizing a small blood sample for diagnosis and surveillance is of great importance. Blood are routinely collected for YF diagnosis and blood clots usually discarded after serum obtention. Aiming to take sample advantage, the sensitivity of a PCR using extracted DNA from long-term frozen clots from human and non-human primates for detection of Plasmodium spp. in low parasitaemia conditions was assayed. Results Malaria diagnosis with DNA extracted from blood clots generated results in agreement with samples obtained with whole blood, including mixed Plasmodium vivax/simium and Plasmodium malariae/brasilianum infections. Conclusion Blood clots from human and non-human primates may be an important and low cost source of DNA for malaria surveillance in the Atlantic Forest. |
| Databáze: | OpenAIRE |
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