Evaluation of colony-based examinations of diarrheagenic Escherichia coli in stool specimens: low probability of detection because of low concentrations, particularly during the early stage of gastroenteritis
| Autor: | Kanji Miki, Yoshio Iijima, Masahiro Toyokawa, Shizuko Kanamori, Shinobu Tanaka, Seishi Asari |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology Feces law health services administration parasitic diseases Escherichia coli medicine Humans Stage (cooking) Escherichia coli Infections Polymerase chain reaction Bacteriological Techniques biology Diarrheagenic Escherichia coli General Medicine biology.organism_classification Enterobacteriaceae Gastroenteritis Diarrhea Infectious Diseases Real-time polymerase chain reaction population characteristics medicine.symptom geographic locations |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 58:303-308 |
| ISSN: | 0732-8893 |
| Popis: | To evaluate traditional colony-based examinations of diarrheagenic Escherichia coli (DEC), we analyzed the proportions of 5 categories of DEC among E. coli in stool specimens from patients with gastroenteritis using real-time polymerase chain reaction with novel primers and probes. Among 81 DEC isolates, 48 (59.3%) were present at proportions ofor = 10%, whereas only 17 (21.0%) reached50%. Low concentrations (or = 10%) of DEC were found, particularly in most (71.8%) stool specimens collected within 48 h after the onset of illness, although such specimens were conventionally collected as close to the time of diarrhea onset as possible. Because the probability of detectingor = 10% DEC by colony-based examinations is very low, traditional laboratory methods might not detect most DEC infections, especially at the start of gastroenteritis. Thus, a diagnosis of DEC infections requires a molecular method that targets not individual colonies but E. coli clusters. |
| Databáze: | OpenAIRE |
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