Oxidative stress-inducible antioxidant adaptive response during prostaglandin F2α-induced luteal cell deathin vivo
| Autor: | Irene Ceballos-Picot, Catherine Garrel, Guy Germain, Kaïs H Al-Gubory |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Glutathione reductase SOD2 Apoptosis Luteal phase Biology Dinoprost medicine.disease_cause Biochemistry Antioxidants Superoxide dismutase Luteal Cells Internal medicine medicine Animals chemistry.chemical_classification Reactive oxygen species Sheep Glutathione peroxidase General Medicine Catalase Adaptation Physiological Up-Regulation Oxidative Stress Endocrinology chemistry biology.protein Female Oxidoreductases Oxidative stress |
| Zdroj: | Free Radical Research. 41:251-259 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.1080/10715760601067493 |
| Popis: | Oxidative stress-induced antioxidant adaptive response would be particularly important to cells in high reactive oxygen species (ROS) environments. We aimed to determine the dynamic adaptive response of antioxidant enzymatic systems in sheep corpus luteum (CL) during PGF2alpha-induced luteal cell death. Activities of superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR), and in situ DNA fragmentation were determined in CL at day 10 of the estrous cycle (0 h) and at 12, 24 or 48 h after PGF2alpha injection. A decrease in plasma progesterone concentration was first observed at 6 h after treatment (P0.05). Apoptotic cells were rarely observed in the CL at 0 h (less than 0.7%), and their incidence increased (P0.01) by 12 h post-PGF2alpha (11.7%) and remained thereafter elevated through 48 h. Activities of SOD1, SOD2, GPX and GSR were not changed at any time points after PGF2alpha treatment. CAT activity increased at 12 h (P0.01) and at 24 h (P0.05) after PGF2alpha treatment as compared to that at 0 h. These findings demonstrate that PGF2alpha induce luteal cell death without depressing the activity of antioxidant enzymes. It is suggested that transient increase in CAT activity is an adaptive response of the CL to oxidative stress induced by PGF2alpha. |
| Databáze: | OpenAIRE |
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