Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
| Autor: | Jennifer R. Honda, Stephanie N. Dawrs, Cody Glickman, Michael J. Strong, L. Elaine Epperson, James L. Crooks, Edward D. Chan, Grant J. Norton, Ravleen Virdi, Melissa E. Lowe, Nabeeh A. Hasan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) nontuberculous mycobacteria Veterinary medicine Pulmonary disease Microbiology Article 03 medical and health sciences 0302 clinical medicine Environmental water Virology parasitic diseases lcsh:QH301-705.5 biology Biofilm food and beverages Species diversity biology.organism_classification rpoB bacterial infections and mycoses 6. Clean water 030104 developmental biology 030228 respiratory system lcsh:Biology (General) Nontuberculous mycobacteria Hawai’i Chimaera (genus) biofilms human activities environment |
| Zdroj: | Microorganisms Microorganisms, Vol 9, Iss 224, p 224 (2021) Volume 9 Issue 2 |
| ISSN: | 2076-2607 |
| Popis: | Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai&rsquo i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai&rsquo i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua&rsquo i (n = 51), O&rsquo ahu (n = 202), Maui (n = 159), and Hawai&rsquo i Island (n = 133) and divided into outdoor (n = 179) or indoor (n = 366) categories. rpoB sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. M. chimaera was frequently identified from respiratory and environmental samples followed by M. chelonae and M. abscessus yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of M. chimaera recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar rpoB gene sequences between all showerhead and respiratory M. chimaera isolates, supporting outdoor and indoor environments as possible sources for pulmonary M. chimaera infections. |
| Databáze: | OpenAIRE |
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