THE COMBINED ESTIMATION OF CORTISOL AND 11-DESOXYCORTISOL IN PLASMA AS PORTER-SILBER CHROMOGENS
| Autor: | Bernadine Serena, Shirley G. Cook, Ernest M. Gold |
|---|---|
| Rok vydání: | 1960 |
| Předmět: |
17-Hydroxycorticosteroids
medicine.medical_specialty Chromatography Hydrocortisone Accurate estimation Endocrinology Diabetes and Metabolism Cortodoxone Biochemistry (medical) Clinical Biochemistry Plasma Biochemistry Porter Silber chromogens 11-Desoxycortisol Solvent chemistry.chemical_compound Endocrinology chemistry Internal medicine Reagent medicine Humans hormones hormone substitutes and hormone antagonists Dichloromethane |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 20:315-326 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-20-2-315 |
| Popis: | Current methods of measuring the concentration of plasma 17-hydroxycorticosteroids, although adequate for cortisol, may vary in the recovery of 11-desoxycortisol. The present study demonstrates that procedures which employ solvent partition to transfer 11-desoxycortisol from the extracting solvent, dichloromethane, into the phenylhydrazine-sulfuric acid-ethanol color reagent lose almost 50 per cent of the steroid in this step. A modification of the Silber-Porter procedure has been developed, which overcomes this difficulty by evaporating the extracting solvent before addition of the color reagent. By the joint quantitation of both cortisol and 11-desoxycortisol this procedure provides a more accurate estimation of total free 17,21-dihydroxy-20-ketosteroids in plasma. The quantitative recovery of 11-desoxycortisol is of considerable practical value, since it is a biosynthetic precursor of cortisol and its presence is being recognized with greater frequency in various spontaneous and induced abnorm... |
| Databáze: | OpenAIRE |
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