Overexpression of stathmin1 in the diffuse type of gastric cancer and its roles in proliferation and migration of gastric cancer cells
| Autor: | Yongseok Lee, Sun-Yong Baek, Hur Gy, Sae-Ock Oh, Sik Yoon, Bongsoo Kim, Geun-Am Song, Hyun-Chul Kim, Jin Young Kim, Jae-Bong Kim, Tae-Yong Jeon, Myoung-Eun Han, Young-Suk Lee, Gwang Ha Kim |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty proliferation Mice Nude Stathmin migration Mice Downregulation and upregulation Cell Movement Stomach Neoplasms Carcinoma medicine Animals Humans Neoplasm Invasiveness RNA Small Interfering Stomach cancer Cells Cultured stathmin1 Aged Cell Proliferation biology Cell growth gastric cancer digestive oral and skin physiology Cancer Cell migration Middle Aged medicine.disease Xenograft Model Antitumor Assays digestive system diseases Up-Regulation Gene Expression Regulation Neoplastic Oncology Case-Control Studies Cancer cell biology.protein Female Translational Therapeutics |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6605537 |
| Popis: | Background: Stathmin1 is a microtubule-regulating protein that has an important role in the assembly and disassembly of the mitotic spindle. The roles of stathmin1 in carcinogenesis of various cancers, including prostate and breast cancer, have been explored. However, its expression and roles in gastric cancer have not yet been described. Methods: Stathmin1 expression in paraffin-embedded tissue sections from 226 patients was analysed by immunohistochemistry. Roles of stathmin1 were studied using a specific small interfering RNA (siRNA). Results: The expression of stathmin1 was positively correlated with lymph node metastasis, TNM stages and vascular invasion, and negatively with recurrence-free survival, in the diffuse type of gastric cancer. The median recurrence-free survival in patients with a negative and positive expression of stathmin1 was 17.0 and 7.0 months, respectively (P=0.009). When the expression of stathmin1 was knocked down using siRNA, the proliferation, migration and invasion of poorly differentiated gastric cancer cells in vitro were significantly inhibited. Moreover, stathmin1 siRNA transfection significantly slowed the growth of xenografts in nude mice. Conclusion: These results suggest that stathmin1 can be a good prognostic factor for recurrence-free survival rate and is a therapeutic target in diffuse-type gastric cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|