Lack of a central role for osteoprogenitor cells from the femoral canal in heterotopic ossification of the hip
| Autor: | Andres Arend, Aare Märtson, Alar Toom, Tiit Haviko, G. Selstam, Siim Suutre |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Osteocalcin Bone Morphogenetic Protein 2 Osteogenesis Bone cell Animals Medicine Orthopedics and Sports Medicine Femur Rats Wistar Saline business.industry Ossification Ossification Heterotopic Stem Cells Femoral canal Anatomy medicine.disease Recombinant Proteins Rats Surgery Disease Models Animal medicine.anatomical_structure Orthopedic surgery Capsulotomy Hip Joint Heterotopic ossification medicine.symptom business |
| Zdroj: | The Journal of Bone and Joint Surgery. British volume. :298-303 |
| ISSN: | 2044-5377 0301-620X |
| Popis: | We have developed an animal model to examine the formation of heterotopic ossification using standardised muscular damage and implantation of a beta-tricalcium phosphate block into a hip capsulotomy wound in Wistar rats. The aim was to investigate how cells originating from drilled femoral canals and damaged muscles influence the formation of heterotopic bone. The femoral canal was either drilled or left untouched and a tricalcium phosphate block, immersed either in saline or a rhBMP-2 solution, was implanted. These implants were removed at three and 21 days after the operation and examined histologically, histomorphometrically and immunohistochemically. Bone formation was seen in all implants in rhBMP-2-immersed, whereas in those immersed in saline the process was minimal, irrespective of drilling of the femoral canals. Bone mineralisation was somewhat greater in the absence of drilling with a mean mineralised volume to mean total volume of 18.2% (sd 4.5) versus 12.7% (sd 2.9, p < 0.019), respectively. Our findings suggest that osteoinductive signalling is an early event in the formation of ectopic bone. If applicable to man the results indicate that careful tissue handling is more important than the prevention of the dissemination of bone cells in order to avoid heterotopic ossification. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|